Autoantibodies against Antigens of the Skin
Indirect Immunofluorescence Test: Dermatology Mosaic 7
- Oesophagus and salt-split skin (top left, middle left): ab against epid. basal membrane. BP230 gc (top right). BP180 (NC16A-4X) (middle right). Desmoglein 1 + 3 (bottom left and right).
- Screening and differentiation test for detection of skin-specific antibodies.
- Indication: bullous autoimmune dermatoses.
- Initial dilution 1 : 10; conjugate class anti-human IgG, FITC-labelled.
- The BIOCHIP Mosaic consists of 6 substrates: primate oesophagus, salt-split skin, desmoglein-1-expressing cells, desmoglein-3-expressing cells, BP230-expressing cells (gc) and BP180 (EUROPLUS). Thus a comprehensive antigen spectrum is available in a single analysis, allowing targeted serological diagnosis.
- Antibodies against prickle cell desmosomes react with surface antigens of keratinocytes. Tissue sections of oesophagus and tongue show a granular fluorescence of the intercellular matter in the whole stratum spinosum, but differentiation between desmoglein 1 and desmoglein 3 is difficult. When specific transfected cells are employed in addition, a targeted diagnosis in a single test run is possible.
- Antibodies against basal membrane structures react with salt-split skin. Anti-BP180, anti-BP230 and anti-LAD97 cause staining of the epidermal side, and antibodies against laminin 5, collagen VII and other antigens staining of the dermal side of salt-split skin.
- When autoantibodies against BP180 or BP230 are present, the epidermal basal membrane in the oesophagus or tongue is visible as a fine linear colouring between the stratum basale and the connective tissue. These antibodies can be differentiated by means of BP180-NC16A-4X coated BIOCHIPs and cells specifically transfected with BP230 (globular C-terminal domain (gC)).
- This BIOCHIP Mosaic can be customised with further substrates if required, e.g. tongue (antibodies against prickle cell desmosomes, epidermal basal membrane), bladder (antibodies against plakins).
| Antigen |
| Order No. |
Oesophagus | FA 1501-#### | ||
Oesophagus/Tongue | FA 1501-####-1 | ||
Tongue | FA 1502-#### | ||
Bladder mucosa | FA 1507-#### | ||
Split skin | FA 150b-#### | ||
Envoplakin | FA 1491-####-50 | ||
Collagen type VII NC1 | FA 1947-####-50 |
Microplate ELISA: Anti-Desmoglein 1, Anti-Desmoglein 3, Anti-Envoplakin, Anti-BP180-NC16A-4X, Anti-BP230-CF
- Incubated ELISA Anti-Desmoglein 1 and 3, Anti-BP180-NC16A-4X, Anti-BP230-CF.
- Monospecific detection of antibodies against desmoglein 1, desmoglein 3, envoplakin, BP180 und BP230.
- Indication: bullous autoimmune dermatoses.
- Serum dilution 1 : 100; conjugate class anti-human IgG, POD-labelled.
- 3-point calibration, quantitative (exception: anti-envoplakin, semiquantitative).
- Identical incubation conditions and times: all tests can be combined on one microplate.
- Recombinant antigens: extracellular domain of desmoglein 1 or 3, envoplakin, tetramer of NC16A domain of BP180 protein, C-terminal segment of BP230 protein. The corresponding human cDNA is produced in E. coli (BP180-NC16A-4X, BP230-CF, envoplakin) or in mammalian cells (desmoglein 1, desmoglein 3).
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