Antibodies against Emerging Viruses

Indirect Immunofluorescence Test

Over the past years it has been observed that a number of new viruses ("emerging viruses") and other pathogens has spread worldwide, introducing since then unknown diseases into previously unaffected regions.
EUROIMMUN offers a broad spectrum of indirect immunofluorescence tests for the detection of specific antibodies (see table).
Many of these substrates are available as single substrate with non-infected cells or as useful combinations (syndrome or geographically orientated) for the investigation of serum samples.
IgG absorption as preparatory step for the determination of specific antibodies of class IgM: page 58.
Cross reactions within the virus family, especially with Flaviviruses, should be taken into consideration since they may cause false-positive results. The infectious agent can be determined by titration of the sample and comparison of titers.

	Pathogen	Disease, syndromes
	Corona virus:
	SARS corona virus (SARS-CoV)	Severe acute respiratory syndrome (SARS)
	Flaviviruses:
	TBE virus (TBEV)	Tick-borne encephalitis
	West Nile virus (WNV)	West Nile fever, encephalitis
	Japanese encephalitis virus (JEV)	Japanese encephalitis
	Yellow fever virus (YFV)	Yellow fever, hepatitis, haemorrhagic fever, arthritis
	Dengue virus (DENV, types 1-4)	Dengue fever, haemorrhagic fever
	Bunyaviruses:
	Hantavirus (types Hantaan, Puumala, Seoul, Saaremaa, Dobrava, and Sin Nombre)	HFRS (haemorrhagic fever with renal syndrome); HCPS (Hantaviral cardiopulmonary syndrome)
	Sandfly fever virus (types Sicilian, Naples, Toscana and Cyprus)	Pappataci fever, meningitis, encephalitis
	Rift valley fever virus (RVFV)	Rift valley fever, haemorrhagic fever, hepatitis
	Crimean-Congo fever virus (CCHFV-GPC and -N)	Crimean-Congo haemorrhagic fever
	Togaviruses:
	Chikungunya virus (CHIKV)	Chikungunya fever, arthritis
	Kinetoplastida:
	Leishmania donovani	Visceral leishmaniasis
	Haemosporina:
	Plasmodium falciparum	Malaria tropica
	Plasmodium vivax	Malaria tertiana

Monospecific determination of antibodies against TBE, West Nile and Dengue virus.
Serum dilution 1: 100, conjugate class anti-human IgG or IgM, POD-labelled.
1-point calibration, semiquantitative (IgM) or 3-point calibration, quantitative (IgG). Similar incubation conditions and times: All tests can be combined on one and the same microplate.
IgM test kit with IgG/RF absorbent in the sample buffer for IgG absorption as preparatory step for the determination of specific IgM antibodies.
Available single ELISA:

Antigen		Order No.
TBE		EI 2661-9601 G or M, avidity, Ab determination in CSF
TBE "Vienna"		EI 2661-9601-9 G
WNV		EI 2662-9601 G or M, avidity
Dengue		EI 266b-9601 G or M