IFA

Indirect immunofluorescence tests (IIFT) from EUROIMMUN

unrivalled quality and variety

Advantages of EUROIMMUN IIFT

  • Unrivalled standardisation of IIFT due to BIOCHIP technology with highest quality on millimetre-sized fragments
  • Combination of BIOCHIPs in Mosaics on one reaction field for multiplex testing
  • Comprehensive product range for autoimmune and infection diagnostics based on BIOCHIPs with cultured cells, antigen dots (EUROPLUS), antigen-expressing cells and tissue sections
  • Test kits containing ready-for-use reagents that are exchangeable between products with bar and colour codes for secure manual and automated processing
  • Various test kit formats for different sample throughputs: slides with 3, 5, 10 and 50 reaction fields
  • Universal incubation schemes for uncomplicated combined processing of different parameters
  • DataMatrix codes on all slides for complete traceability
  • Brilliant fluorescence images due to incubation using TITERPLANE technology

  • Test principle

    In the first incubation step, specific antibodies from the diluted patient sample bind to the solid-phase bound antigens. In the next step, a fluorescein (FITC)-labelled antibody (conjugate) binds to the specific antibodies from the patient sample. By excitation with the respective wavelength, the complex can be made visible at the fluorescence microscope. The test processing is manual, semi- or completely automated.


    BIOCHIP technology

    The BIOCHIP technology developed by EUROIMMUN allows unrivalled quality and variety in IIFT. Due to a special activation technique, cover glasses are modified in such way that very different substrates can be coated with highest adhesion. Cultured cells, tissue sections, antigen-expressing cells and purified antigens (antigen dots) are used as substrates. The cover glasses coated with biological substrates are cut mechanically into millimetre-sized fragments (BIOCHIPs). Many preparations with consistent, high quality can be obtained per tissue section, for cultured cell substrates even several thousands. The BIOCHIPs are fixed precisely on the test fields of specially developed plastic slides. When multiple BIOCHIPs coated with different substrates are arranged in one reaction field, antibodies against various organs or infectious agents can be investigated simultaneously. Such BIOCHIP mosaics allow the generation of comprehensive antibody profiles (multiplex) with minimal sample volumes or the reciprocal verification of the results on different substrates.


    TITERPLANE technology

    BIOCHIP technology not only ensures highest standardisation of substrate production but also, together with TITERPLANE technology, provides a unique form of incubation for brilliant fluorescence images.

    In the TITERPLANE technique, the diluted samples are first pipetted onto the fields of a glass reagent tray. The fields of the reagent tray are hydrophilic while the area outside the fields is hydrophobic, so that the samples cannot run into each other. Only when all the samples have been pipetted are the slides placed onto the reagent trays, with the BIOCHIPs facing downwards. In this way, all reaction fields come into contact with the samples at the same time and the reactions are started simultaneously for all samples. This prevents deviations in the reaction intensity due to differences in the incubation times. 

    Another advantage of TITERPLANE technology and of placing the slides onto the samples from above results from gravity: Crystals or particles in the samples do not accumulate on the BIOCHIPS, but on the reagent tray. Moreover, TITERPLANE technology prevents evaporation of the samples, rendering a conventional “humidity chamber” unnecessary. This results in optimal fluorescence images which can be evaluated visually or fully automatically.


  • Automation

    EUROLabWorkstation IFA

    Product

    MERGITE!

    Product

    IF Sprinter

    Product

    Sprinter XL

    Product

    EUROStar III Plus

    Product

    EUROPattern Microscope Live

    Product

    EUROPattern

    Product

Techniques

Immunoblot

Technique

EUROArray

Technique

EURORealTime

Technique

Radioimmunoassays

Technique
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